Antimicrobial activity of solvent extracts and flavonoids of calotropis procera growing wild in saudi arabia was evaluated using the agar welldiffusion method. Shyamala and thangaraju 20 revealed the presence of the secondary metabolites viz. Therefore, combining advance pharmacological and traditional knowledge. Antimicrobial screening and brine shrimp lethality. Approximately 850 gm of the powder of whole plant of calotropis gigantea was soaked with ethanol and. Antimicrobial activity of latex of calotropis gigantea against pathogenic microorganisms an in vitro study article pdf available in pharmacologyonline 3. The root and latex of calotropis gigantea were screened for its antimicrobial and phytochemical activities. Antimicrobial activities evaluation from the extracts of. In vitro anticandida activity of calotropis gigantea against clinical isolates of. Calotropis gigantea and calotropis procera are to a great extent having a very similar chemical properties, but the colours of their flowers are different. Antioxidant, antimicrobial activities and gcms analysis. Purification and characterizations of antimicrobial protein from.
To evaluate antimicrobial and phytochemical activity of hexane and ethanolic extract of ocimum sanctum and calotropis gigantea leaves on clinical pathogens. Antioxidant activity of crude extract and latex inhibition ranging from 1220mm while latex was found extracted from the calotropis procera more active which showed activity with inhibition zone fig. Antibacterial activity of crude extract and latex crude was 20. Received application of latex of calotropis gigantea 200 mgkgday wound healing activity excision and incision wound models were used to evaluate the woundhealing activity of latex of c alotropis gigantea. Calotropis gigantea latex, antifungal activity, minimum fungicidal concentration, zone of inhibition, disc diffusion method correspondence to author. Antioxidant, antimicrobial activities and gcms analysis of calotropis gigantea white flowers shopna rajamohan, prabakaran kalaivanan, ilayaraja sivagnanam, manivannan rajamanickam abstract calotropis gigantea white asclepiadaceae, is a weed plant commonly known as giant milkweed. Antibacterial activity well plate method was employed on leaf extract of calotropis gigantea against certain gram positive b. Microwave assisted extraction and evaluation of herbal plant extract. Antimicrobial activity and phytochemical analysis of calotropis gigantea root, latex extracts.
The antibacterial activity therefore shows clearly that the resistance to the pathogens may be minimized by these natural products of the plant origins. In the present study leaf extracts of withania somnifera, calotropis procera, tinospora. Phytochemical analysis, antibacterial and antioxidant. Calotropis gigantea linn and calotropis procera ait 5. The ph of latex of these two species has been found different in the present study. Approximately 675 segments from 15 calotropis procera plants and 15 latex samples were assessed for the presence of endophytic fungi. Antibacterial, antioxidant, calotropis gigantea, calotropis procera, dpph assay, ftir, gcms, total phenolic content. Antimicrobial and cytotoxic activity of di 2ethyl hexyl phthalate and anhydrosophoradiol 3acetate isolated from calotropis gigantea linn. Antimicrobial properties of ocimum sanctum and calotropis. Pdf antibacterial and phytochemical studies on calotropis. Medicinal plants are having immense potential to cure various health ailments and used as drugs and remedies. Phytochemical and antimicrobial evaluation has been carried out on other species of calotropis. This plant is popularly known because it produces large quantity of latex 1. Calotropis procera belongs to the family aselepiadaceae.
Antimicrobial activity of green silver nanoparticles from endophytic. Antibacterial activities and mic value of different crude extracts of c. Plant is one of the important source of drugs thats why we have done this project work to find out some biological activities of the plant exudates of calotropis gigantea where antimicrobial, cytotoxic and antioxidant activity were analyzed. Phytochemical investigation of different plant parts of. The agar diffusion test was used to check the antimicrobial activity of the hexane and ethanolic extracts of the medicinal plants. The study was approved by the institutional animal ethical committee of b. Therefore, these plants have merits for further screening on. Result of the antimicrobial activity of root extracts of calotropis gigantea. Calotropis gigantea belonging to the family asclepiadaceae has been investigated for the presence of its extracts with special emphasis to the brine shrimp lethality bioassay, thrombolytic activity and antimicrobial activity. Green synthesis of silver nanoparticles using calotropis. In vitro antibacterial activity of medicinal plants. The antibacterial activity studies of these plants are still limited especially on the bacteria that cause caries. Inhibition of fungal stain by calotropis procera latex.
Pdf antimicrobial activity and phytochemical analysis of. Therefore the present study was designed to investigate antimicrobial and cytotoxic activity leave extract of calotropis gigantean. Antibacterial and cytotoxic potential of silver nanoparticles synthesized using latex of calotropis gigantea l. Ms is an analytical technique which merge the features of. Calotropis gigantea, antimicrobial activity, cytotoxicity. Evaluation of anti microbial activity and phytochemical. Antifungal, antioxidant and antibacterial activities of. To test of antimicrobial activity, disc diffusion method has been followed. Antimicrobial activity assay done against bacterial and fungi. Calotropis gigantea crude extract, phytochemicals, antimicrobial activity. Paper open access the potential of five therapeutic. Calotropis gigantea for the antimicrobial activity against clinical isolates of bacteria. Pdf antimicrobial activity of calotropis procera ait. Varahalarao et al 2010 examined bioassays for antimicrobial activities using stem, leaves and flowers of calotropis procera.
Although calotropis gigantea have various ethnopharmacological uses, the plant have not been undergone any chemical and pharmacological investigation. It belongs to the family of asclepiadaceous, habitat of india, indonesia, sri lanka and china. Screening and gcms profiling of ethanolic flower extract of calotropis gigantea linn. Antimicrobial activity and phytochemical analysis of. From the results it was evident that the flower of vinca rosea showed the highest antioxidant activity of 97. Preliminary phytochemical screening of calotropis gigantea.
The aim of the present study was to screen leaves of calotropis gigantea for the antimicrobial activity against clinical isolates of bacteria. Antibacterial, calotropis, leaves, microscopical, phytochemical. Antimicrobial activity of latex of calotropis gigantea against pathogenic. Phytochemical and pharmacological investigation of. Calotropis gigantea called swetarka and calotropis. Antimicrobial activities evaluated from leaves, flowers, fruits and latex of locally obtained calotropis procera from alsharifieya, taif in different aqueous solutions and solvents such as sterilized zamzam water and distilled water, ethanol, acetone. Maximum activity was seen in case of pseudomonas fluorescens where the zone diameter was 32 mm 300. It has clusters of waxy flowers that are either white or lavender in colour.
The phytochemical screening revealed the presence of alkaloids, flavonoids, tannins, saponins, and cardiac glycosides with a very high content in water extracts. Preliminary phytochemical, antimicrobial and photochemical study of calotropis gigantea leaf extract, pages. Review on antibacterial activity of himalayan medicinal plants. The plant is erect, tall, large, much branched and perennial with milky latex throughout. Phytochemical and pharmacological studies of the whole. Calotropis a medicinal plant of family asclepiadaceae has been utilized in ayurveda, unani, siddha and many other traditional systems to cure diseases. Several plant products have been found to be a part of phytomedicines since time immemorial. Calotropis gigantea is a common wasteland weed and known for various medicinal properties.
Maximum activity was conferred against eschershia coli 4mm and shigella 3. Br asclepiadaceae is a xerophytic, erect shrub, growing widely throughout the tropical and subtropical regions of asia and africa. These plant products can be derived from barks, leaves, flowers, roots, fruits, and seeds. Antimicrobial activities of calotropis procera on selected. The antimicrobial effect of ethanol, aqueous and chloroform extracts of leaf and latex of calotropis procera on six bacteria namely, escherichia coli.
The species of calotropis gigantea has flowers ranging from purple to white colour. Antibacterial activity of aqueous extract of calotropis gigantea leaves an in vitro study. Antimicrobial activity of latex of calotropis gigantea against pathogenic microorganisms an in vitro study gaurav kumar, loganathan karthik and kokati venkat bhaskara rao molecular and microbiology research lab, environmental biotechnology division, school of bio science and technology, vit university, vellore632 014, tamil nadu, india. Phytochemical and antibacterial activities of water, methanol and ethanol extracts obtained from the fruit and bark of calotropis procera were investigated in an attempt to evaluate its medicinal potentials. Phytochemical and antibacterial properties of calotropis. Review on antibacterial activity of himalayan medicinal plants traditionally. Calotropis gigantea, the crown flower, is a species of calotropis native to cambodia, indonesia, malaysia, the philippines, citation needed thailand, sri lanka, india, china, pakistan, nepal, and tropical africa it is a large shrub growing to 4 m ft tall.
Chitme hr, chandra r, kaushik s 2004 studies on antidiarrhoeal activity of calotropis gigantea r. The different parts of calotropis gigantea and vinca rosea belonging to the families of asclepiadaceae and apocynaceae were studied for their antioxidant and antimicrobial activities against selected bacterial strains. Phytochemical and antimicrobial evaluation of aqueous and organic extracts of calotropis procera ait leaf and latex. Antibacterial activity of aqueous extract of calotropis. Calotropis procera is strongly recommended in leprosy, hepatic and. Calotropis gigantea ethyl acetate extract was used for the isolation of 1di2ethylhexyl phthalate compound 1. Calotropis procera extracts against seedborne fungi agrees with the work of abdulmoniem et al. Different parts of calotropis procera have been reported to exhibit medicinal and nutritional properties while. Antimicrobial activity and phytochemical analysis of calotropis. Invitro antimicrobial activities and phytochemical. Antimicrobial and cytotoxic activity of di2ethylhexyl. Study of methanolic extract of leaves calotropis gigantea. But a few of isolated compounds from calotropis gigantea and amoora rohituka have potent antineoplastic, antimicrobial and insecticidal activities. Antimicrobial activities of different parts of calotropis.